![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fsrep12543/MediaObjects/41598_2015_Article_BFsrep12543_Fig1_HTML.jpg)
Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports
![SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly on length and composition of the primer(s). An annealing temperature 5°C SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly on length and composition of the primer(s). An annealing temperature 5°C](https://cdn.numerade.com/ask_images/4923a58bd9124b0c9cc690a3580c29e8.jpg)